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Mixed microbe cultureGENERAL MICROBIOLOGY LAB
BIOLOGY 208 Tentative Syllabus: Thompson Complex, North Wing, Room 200: (see your instructor's syllabus below)

This course fulfills the D.1. (Science/Mathematics) general education requirement and should be taken concurrently with Biology 207. It will help you attain this general education goal and its corresponding
objectives: An understanding of the scientific method and a knowledge of natural science and its relevance in our lives, specifically:

1. How scientific knowledge is created, developed, and changed through experimentation and reasoning,
2. Demonstration of knowledge in one or more of the sciences, including theories, concepts, and
principles that explain observations and make predictions,
3. How to locate and evaluate reliable resources to acquire information about scientific developments, and the outlining of the reciprocal relationship between humans and the rest of the ecosystem.

PREREQUISITES: No prerequisites are required for this course but introductory knowledge of biology and chemistry would be beneficial.

OBJECTIVES: To introduce and provide experience with some fundamental laboratory techniques necessary for the study of microbes.

LAB MANUAL: Microbiology Laboratory for the Health Science Student; a Clinical Approach. First Edition, All students are required to purchase or download (see below) a new laboratory manual. Using previously used lab manuals is not permitted.

ONLINE LAB MANUAL: Files are in PDF format suitable for printing The files can be opened with your browser (Internet Explorer or Netscape) and the free Adobe Reader.

OTHER MATERIALS:

ATTENDANCE: Every laboratory period must be attended punctually and entirely (See section on GRADING).

EXAMS: Three exams will be given. Exam I (50 points) will be given during the first hour of period 5 and will cover material from labs 1-4. Exam II (50 points) will be given the first hour of or prior to period 9 and will cover material from labs 5-7. Exam III (100 points) will be given during Period 15 and cover material from labs 8-13. Exams II and III Review. http://bioweb.wku.edu/faculty/Clauson

Exam Reviews can also be found at. http://bioweb.wku.edu/faculty/Clauson

GRADING: Each student's Term Letter Grade (TLG) will be determined by the scores earned on the three exams (200 total points) plus credit for each of 13 laboratory periods in which each student is required to complete and record exercise data. The instructor reserves the right to randomly request your laboratory data pages to assure their completion and evaluate performance. Four (4) points will be earned after completing each laboratory period and recording the data from that period in your laboratory manual.

These points will be summed to obtain a Cumulative Score. The maximum number of points awarded will be 150 (test scores) + 44 (11 completed laboratories) = 194.

Basis for Assigning each TLG

175 points A
155 points B
135 points C
115 points D
Below 115  F

REQUIRED MATERIALS:
Students are required to have an acceptable form of laboratory outerwear. The current policy regarding laboratory outerware is as follows: If you are aware of any accident, spill or contamination to your laboratory outerware, the garment is to be sterilized before it leaves the facility.
Students working with laboratory staining reagents are required to wear ANSI Z87.1-1989 high impact eye protection. - see schedule for designated lab exercises (*).

DISABLILITIES: "Students with disabilities who require accommodations (academic adjustments and/or auxiliary aids or services) for this course must contact the Office for Student Disability Services, Room 445, Potter Hall. The OFSDS telephone number is (270) 745-5004 V/TDD.

SCHEDULE: Consult your Instructor's Syllabus for schedule details.

CRN Class Instructor Syllabus link

Tentative Schedule
- The schedule for your schedule may vary
Lab  Exercise
1   Orientation, Laboratory Safety
1. The Microscope
2. Handling and Examining Cultures
2  3. The Wet-Mount Preparation
4. Simple Stains
3   5. *Gram stain
6. *Special stains Endosporestain
7. Pure culture technique
4   9. Biochemical Reactions, Sulfate Indole Motility
10. Enzymatic Reactions, Catalase Test
11. Enzymatic Reactions, Oxidase Test
12. Enzymatic Reactions, Urease Test
13. Enzymatic Reactions, Deaminase Test
5  14. Antibiotics, Susceptibility Testing, MIC
15. Primary Media for Isolation of Microorganisms
16. Isolation of Pathogenic Yeasts
6 Exam 1 (Labs 1 - 4)
7  *   

17. Alpha Hemolytic Streptococci
18. Beta Hemolytic Streptococci
19. *Enterococci
20. Staphylococci
21. Streptococci from a throat culture

8  *      17. Alpha Hemolytic Streptococci
18. Beta Hemolytic Streptococci
19. *Enterococci
20. Staphylococci
21. Streptococci from a throat culture
9  * 26. *Dip Stick Urine Test
27. *Qualitative Urine Culture
28. *Quantitative Urine Culture
29. Neisseria
30. Spirochetes
10 EXAM II (Labs 5 - 8)
11 *     31. *Clostridium, endosprulation patterns
32. Culturing Anaerobic Bacteria
33. *Mycobacteria, Acid Fast Stain
12  * 34. Differentiation, Gram Negative Rods
35. Rapid Methods for Identification
36. Pathogenic Molds and Dermatophytes
13  * 37. Serologic Identification of Microorganisms
38. Serologic Identification of Patients Antibodies
14 REVIEW
15  FINAL EXAM (Labs 9 - 13)

Link to WKU HomePage Link to WKU Biology Home Page (Bioweb) * Laboratory exercises require eye protection

Bioweb

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